Magnetically Recoverable δ‑FeOOH Particles for Multilayer Enzyme Immobilization and Surface-Induced Activity Tuning.

ABSTRACT: Enzyme immobilization is an effective strategy to enhance enzyme stability, which remains a major drawback in biocatalysis. However, achieving high enzyme loading without loss of activity is still a key limitation of conventional support. Most supports that overcome these limitations, such as nano- and microparticles, involve high production costs. Therefore, a support that combines the advantages of high surface area, easy recovery, and low-cost production is still lacking in the literature. In this study, we present a low-cost, dual-function, magnetically recoverable support material, superparamagnetic δ-FeOOH (feroxyhyte) particles, that enhances enzyme activity and modulates protein structure both prior to, and following immobilization. We show that δ-FeOOH enables lipase immobilizatio

SUBMITTER: Almeida FLC 

PROVIDER: S-EPMC12713457 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature