Development of a Multiplex qPCR to Detect and Identify <i>Cryptococcus neoformans</i> and <i>Cryptococcus gattii</i>.

ABSTRACT: Background: Atypical clinical symptoms often lead to missed diagnosis or misdiagnosis of cryptococcal infection. Cryptococcus neoformans and Cryptococcus gattii exhibit similar clinical manifestations but different treatment cycles and prognoses. Methods: A multiplex qPCR was developed to detect and identify two fungi, which are based on the IGS-1 region gene of Cryptococcus neoformans (Cn-IGS-1) and Cryptococcus gattii (Cg-IGS-1), and ribonuclease P (RNP) gene. The amplification efficiency, precision, specificity, and detection sensitivity were determined. The detection sensitivity of the multiplex qPCR was compared with Indian ink staining, culture, and lateral flow immunochromatographic assay (LFA). The impact of standing time on t

SUBMITTER: Ye WM 

PROVIDER: S-EPMC12713477 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature