Investigation of TRMT61B methyltransferase activity on mRNA and its effects on translation.

ABSTRACT: Despite recent advances in technology to map RNA chemical modifications transcriptome-wide, the distribution of N¹-methyladenosine (m¹A) in mRNA remains contested, hindering a clear understanding of its function. Additionally, the enzyme(s) that installs the majority of reported mRNA m¹A sites has yet to be identified. In this study, we characterized TRMT61B, an m¹A methyltransferase known to methylate mitochondrial RNAs, but whose sequence preferences have been underexplored. By integrating cellular overexpression of TRMT61B and in vitro methylation of a synthetic pool of diverse human RNA sequences, we identified a preference for a YMRA consensus motif in single stranded RNA regions. In these experiments, TRMT61B methylated thousands of

SUBMITTER: Fang D 

PROVIDER: S-EPMC12713599 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature