Dissecting Heterogeneous Populations of Protein-Complex Samples Using Direct Mass Technology.

ABSTRACT: Charge detection mass spectrometry (CDMS) is an emerging technique for the qualitative analysis of heterogeneous biological samples by individually measuring the charge and m/z of ions to determine their mass. The commercialization of CDMS with Orbitrap-based CDMS has allowed for broader use of the technique within the native mass spectrometry (nMS) field. However, CDMS is still primarily used qualitatively, with only a few studies applying it for quantitation in the MDa range. Here, we demonstrate how Orbitrap-based CDMS/Direct Mass Technology (DMT) can be used for quantitation of heterogeneous transthyretin (TTR) tetramer samples <100 kDa. First, we demonstrate DMT can resolve wild-type (WT) and C-terminally tagged (CT) TTR homotetramers and hybrid tetramers (i.e., tetramer

SUBMITTER: Rider RL 

PROVIDER: S-EPMC12713611 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature