Relative contributions of the ERG11^VF125AL and MRR1A^N647T mutations to fluconazole resistance in Clade III Candidozyma (Candida) auris clinical isolates.

ABSTRACT:

Objectives

Candidozyma (Candida) auris is an emerging fungal pathogen of global concern that often exhibits multidrug resistance. Of the six described C. auris clades, Clade III has been found to be nearly universally fluconazole resistant, and almost every Clade III isolate described carries a mutation leading to a VF125AL substitution in ERG11, the gene encoding the fluconazole target lanosterol 14α-demethylase, and a mutation leading to a N647T substitution in the gene encoding Mrr1a, a transcriptional regulator of the Mdr1 transporter. In this study, we aimed to determine the specific contributions of these mutations to triazole antifungal susceptibility in Clade III C. auris isolates.

Methods

We introduced the Clade III MRR1A mutation into a Clade I background using CRI

SUBMITTER: Barker KS 

PROVIDER: S-EPMC12713649 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature