Age-specific genomic and transcriptomic variation reveals limited evidence for <i>cis-</i>regulatory interactions modulating aging in <i>Saccharomyces cerevisiae</i>.

ABSTRACT: The budding yeast Saccharomyces cerevisiae is a well-established model for studying the genetic basis of complex traits, and it is a powerful system for investigating mechanisms of aging. Here, we examine the genomic and transcriptomic factors contributing to increased replicative age in recombinant yeast populations harboring standing genetic variation. Using Fluorescence-Activated Cell Sorting (FACS), we isolated young and aged cohort pairs across twelve biological replicates and sequenced their progeny to assess patterns of differentiation at the nucleotide and transcription levels. Most differentiated alleles were located in coding regions, including significant variants within 132 unique genes. Transcriptomic analysis revealed 60 differentially expressed genes in aged populatio

SUBMITTER: McHugh KM 

PROVIDER: S-EPMC12713674 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature