Functional synergy and genomic linkage of glyphosate resistance traits in Canada fleabane.

ABSTRACT:

Background

Glyphosate resistance in Conyza canadensis (Canada fleabane) has been primarily attributed to non-target-site resistance (NTSR) mechanisms such as vacuolar sequestration, though these have not been formally elucidated. While a target-site mutation at EPSPS2 (P106S) was recently identified, it failed to account for many resistant cases. These findings underscore the need to re-evaluate the genetic basis of glyphosate resistance in this species.

Results

Using an F₂ population derived from glyphosate-resistant and susceptible biotypes, we disentangled the individual and combined effects of target-site resistance (TSR) and NTSR. Dose-response phenotyping and genotyping revealed that NTSR conferred broad protection across a wide range of glyphosate doses, wh

SUBMITTER: Page ER 

PROVIDER: S-EPMC12713706 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature