RamanOmics Decodes Spatial Vibrational-Molecular Architecture and Rewiring in Aging and Repair.

ABSTRACT: Aging and tissue repair involve multilayered and spatially heterogeneous remodeling across transcriptional, biochemical, and cellular dimensions, yet prevailing definitions rely on isolated molecular markers that obscure how biochemical and transcriptional states co-evolve in tissues. Here we present RamanOmics, a multimodal framework that integrates single-nucleus RNA sequencing (snRNA-seq), spatial transcriptomics, and label-free Raman imaging to map the spatial vibrational-biochemical and molecular architecture of aging and senescence directly in intact tissues. Applied to mouse lung and skin, RamanOmics generates spatially resolved biochemical-molecular maps revealing tissue-specific programs: lung senescent cells are enriched for extracellular matrix (ECM) remodeling and TGF-β signali

SUBMITTER: Zhang K 

PROVIDER: S-EPMC12713760 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature