Landscape Expansion Microscopy Reveals Interactions between Membrane and Phase-Separated Organelles.

ABSTRACT: Landscape Expansion Microscopy (land-ExM) is a light microscopy technique that visualizes both lipid and protein ultrastructural context of cells. Achieving this level of detail requires both superresolution and a high signal-to-noise ratio. Although expansion microscopy (ExM) provides superresolution, obtaining high signal-to-noise images of both proteins and lipids remains challenging. Land-ExM overcomes this limitation by using self-retention trifunctional anchors to significantly enhance protein and lipid signals in expanded samples. This improvement enables the accurate visualization of diverse membrane organelles and phase separations, as well as the three-dimensional visualization of their contact sites. As a demonstration, we revealed triple-organellar contact sites among the stres

SUBMITTER: Zhuang Y 

PROVIDER: S-EPMC12714020 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature