Effects of microtubule (de)tyrosination on the morphology and motility of Trypanosoma brucei and cross-talk with polyglutamylation.

ABSTRACT: Post-translational modifications (PTMs) of microtubules control many aspects of their functionality. Specifically, the C-terminal tails of α- and β-tubulin harbour a complex array of PTMs, including polyglutamylation and the reversible detyrosination/tyrosination. The spatial proximity of these two distinct sets of PTMs suggests the possibility of a functional cross-talk between polyglutamylation and (de)tyrosination. In this study, we employ gene deletion and overexpression of the enzymes tubulin-tyrosine ligase (TTL) and tubulin-tyrosine carboxypeptidase (VASH) to provide a detailed analysis of the effects of (de)tyrosination on the protozoan parasite Trypanosoma brucei. While the deletion of either of the enzymes is not lethal, cells exhibit subtle morphological defects, resulting from

SUBMITTER: Thein M 

PROVIDER: S-EPMC12714142 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature