Integrated transcriptomic analysis reveals a CEBPB-DUSP1 axis driving tumor progression in colorectal cancer.

ABSTRACT: Colorectal cancer (CRC) is a prevalent and lethal malignancy, yet the transcriptional networks driving its progression remain incompletely defined. Here, we used integrated transcriptomic analyses of healthy and colorectal cancer samples from three datasets-TCGA-COAD, GSE100179 and GSE196006, and laboratory assays to uncover CCAAT/enhancer-binding protein beta (CEBPB) as a key driver of CRC. Elevated CEBPB expression in clinical samples correlated with shorter overall survival, suggesting its utility as a prognostic marker. We next identified DUSP1, a dual specificity phosphatase critical for MAPK regulation, as a direct transcriptional target of CEBPB. Motif enrichment and promoter scans revealed three high-affinity CEBPB-binding sites in the DUSP1 promoter. Luciferase reporter assays con

SUBMITTER: Zhou H 

PROVIDER: S-EPMC12714181 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature