The master virulence regulator PhoP dictates carbon metabolism by controlling cyclic AMP synthesis in Salmonella.

ABSTRACT: The intracellular pathogen Salmonella enterica serovar Typhimurium confronts cytoplasmic Mg2+ starvation inside macrophages. This stress alters carbon metabolism and subverts canonical carbon source preferences by reducing synthesis of 3', 5'-cyclic adenosine monophosphate (cAMP), the essential allosteric activator of the cAMP receptor protein (CRP), master regulator of carbon utilization. How, then, does S. Typhimurium preferentially utilize CRP-cAMP-dependent carbon sources inside macrophages? We now report that the virulence and Mg2+ homeostasis regulator PhoP controls CRP-cAMP-dependent transcription, metabolism, and growth on a mixture of carbon sources during low cytoplasmic Mg2+. We determine that the PhoP-activated MgtA and MgtB proteins promote CRP-cAMP activity by importing Mg2+,

SUBMITTER: Pokorzynski ND 

PROVIDER: S-EPMC12714265 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature