High analytical variability exists with multiple methods of serum 25-hydroxyvitamin D analysis in dogs.

ABSTRACT: Vitamin D deficiency, defined as low serum 25-hydroxyvitamin D (25D), is a highly prevalent finding in dogs with several disease states. Significant analytical variability between 25D methods of analysis in humans is well-documented; it is unknown if this variability exists in dogs. Our primary goal was to evaluate agreement of 2 chemiluminescent immunoassays (CLIA1, CLIA2); 2 liquid chromatography with tandem mass spectrometry (LC-MS/MS1, LC-MS/MS2) assays, from 3 laboratories; and a point-of-care lateral flow assay (LFA) across a wide range of expected 25D results in dogs. The null hypothesis was that all methods of analysis would have clinically acceptable agreement (<25 nmol/L difference). The tests were assessed for agreement via Bland-Altman analysis, Passing-Bablok regression, and L

SUBMITTER: Reynolds LJ 

PROVIDER: S-EPMC12714551 | biostudies-literature | 2026 Mar

REPOSITORIES: biostudies-literature