^hInGeTox: a human-based in vitro platform to evaluate lentivirus/host interactions that contribute to genotoxicity.

ABSTRACT: Lentivirus vectors are effective for treatment of genetic disease. However, safety associated with vector related genotoxicity is of concern and currently available models are not reliably predictive of safety in humans. We have developed ^hInGeTox as the first human in vitro platform that uses induced pluripotent stem cells and their hepatocyte like cell derivatives to better understand vector-host interactions that relate vectors to their potential genotoxicity. Using lentiviral vectors carrying the eGFP expression cassette under SFFV promoter activity, that only differ by their LTR and SIN configuration, we characterised vector host interactions potentially implicated in genotoxicity. To do this, lentiviral infected cells were subjected to an array of assays and data from thes

SUBMITTER: Suleman S 

PROVIDER: S-EPMC12714580 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature