Novel ubiquitination-related biomarkers for Crohn's disease identified by multi-omics study and experimental validation.

ABSTRACT:

Background

Ubiquitination plays a critical role in the onset and progression of Crohn's disease (CD). Nevertheless, the role of ubiquitination-related genes (URGs) in CD remains incompletely characterized. This study aimed to identify URGs with diagnostic value in patients with CD and construct a URGs-based diagnostic model for CD.

Methods

Single-cell and bulk RNA sequencing datasets related to CD were retrieved from the Gene Expression Omnibus (GEO) database. Single-cell analysis was conducted to characterize cell subsets associated with ubiquitination processes. CellChat was employed to elucidate potential intercellular communication networks, providing insights into the signaling interactions among different cell subsets. Furthermore, High-dimensional weighted gene co-exp

SUBMITTER: Hu J 

PROVIDER: S-EPMC12714605 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature