Pig regulatory macrophages as a donor-derived immune modulators in xenotransplantation.

ABSTRACT:

Introduction

Xenotransplantation offers a potential solution to the critical shortage of donor organs; however, graft survival is limited by xeno-immune activation and coagulation dysregulation. Regulatory macrophages (Mregs) are known for their immunomodulatory capacity, yet their cross-species function in the context of xenotransplantation remains unclear. This study investigates the immunoregulatory properties of pig-derived Mregs (pMregs) as a potential donor-derived cellular therapy.

Methods

pMregs were generated from pig CD14⁺ monocytes using M-CSF and IFN-γ. Phenotypic characterization was performed by flow cytometry, and functional assays evaluated cytokine secretion, T-cell suppression, and induction of FOXP3⁺ regulatory T cells across pig, monkey, and human lymphoc

SUBMITTER: Vu PC 

PROVIDER: S-EPMC12714666 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature