Emodin inhibits colon cancer tumor growth by suppressing tumor cell glycolysis through inhibition of NAT10-mediated PGK1 ac4C modification.

ABSTRACT:

Introduction

Inhibition of glycolysis represents a potent therapeutic approach for colon cancer. Emodin, a natural plant-derived compound with anticancer properties, has shown efficacy in cancer treatment. NAT10 promotes cancer progression by catalyzing ac4C production on mRNAs, but it remains unclear whether emodin regulates glycolysis in colon cancer cells by targeting NAT10. This study aimed to investigate the role of emodin in glycolysis regulation in colon cancer and its underlying mechanisms.

Methods

Glycolysis was assessed by measuring cell proliferation, glucose uptake, lactate production, and extracellular acidification rate. The ac4C levels and NAT10 expression were measured by dot blot and quantitative real-time PCR. The underlying mechanism was investigated by me

SUBMITTER: Wei Z 

PROVIDER: S-EPMC12714669 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature