MRNA vaccines encoding variant forms of Sm-TSP-2 confer protective immunity against Schistosoma mansoni.

ABSTRACT: This study explored the development and evaluation of mRNA vaccine candidates targeting Schistosoma mansoni tetraspanin-2 (Sm-TSP-2). We designed constructs encoding either full-length Sm-TSP-2, or its large extracellular loop (EC2) domain in secretory, membrane-anchored, or cytosolic forms. In a murine model, the secreted and membrane-anchored versions of Sm-TSP-2-EC2 induced the highest antigen-specific antibody titers. These two construct designs, along with full-length Sm-TSP-2 mRNA, also significantly reduced adult worm and egg burden compared to controls. The membrane-anchored Sm-TSP-2-EC2 mRNA was the most effective, lowering worm and egg burdens by 66.7% and 66.9%, respectively. Protective responses induced by the mRNA vaccines were comparable to those elicited by the Sm-TSP-2-EC2

SUBMITTER: de Oliveira AS 

PROVIDER: S-EPMC12714763 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature