Real-time wide-field fluorescence lifetime imaging via single-snapshot acquisition for biomedical applications.

ABSTRACT: Fluorescence lifetime imaging (FLI) is a powerful tool for investigating molecular processes, microenvironmental parameters, and molecular interactions across tissue to (sub-)cellular levels. Despite its established value in biomedical applications, conventional FLI techniques suffer from long acquisition times, limiting their utility in real-time scenarios like fast biological processes and rapid clinical image-guided interventions. Here, we introduce a novel FLI approach that achieves real-time capability through single-snapshot acquisitions by combining a large-format time-gated SPAD array with dual-gate acquisition capability and a rapid lifetime determination algorithm, thus eliminating time-consuming temporal data collection. We demonstrate this method's scalability and versatility a

SUBMITTER: Pandey V 

PROVIDER: S-EPMC12714819 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature