Functional profiling and visualization of the sphingolipid metabolic network in vivo.

ABSTRACT: Sphingolipids govern diverse cellular processes; their dysregulation underlies numerous diseases. Despite extensive characterizations, understanding the orchestration of the sphingolipid network within living organisms remains challenging. We established a versatile genetic platform of CRISPR-engineered reporters of 52 sphingolipid regulators, recapitulating endogenous gene activity and protein distribution. This platform further allows conditional protein degradation for functional characterization. In addition, we developed the biosensor OlyA^w to detect ceramide phosphoethanolamine and visualize membrane raft dynamics in vivo. Using this platform, we established comprehensive profiles of the sphingolipid metabolic network in the brain at the transcriptional and translational l

SUBMITTER: Tzou FY 

PROVIDER: S-EPMC12714868 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature