Screening and identification of human HPV18VLP neutralizing antibodies from women receiving HPV vaccine.

ABSTRACT: Currently, the primary treatments for cervical cancer patients are surgery or chemoradiotherapy, with a lack of standardized pharmacological therapies. Here, we report the construction of a Fab fragment phage display antibody library with a capacity of 10^9, derived from peripheral blood mononuclear cells of 15 women vaccinated against HPV. Through four rounds of screening, one clone, VLP18-Fab, specifically binding to HPV18 virus-like particles (HPV18 VLP), was selected from 40 clones. Additionally, the VLP18-Fab antibody demonstrated a highly significant and dose-dependent neutralizing activity against HPV18 pseudoviruses (P < 0.0001).Moreover,following identification, the structure of the antibody-antigen binding complex was simulated using BIOVIA Discovery Studio, which included the id

SUBMITTER: Gu Y 

PROVIDER: S-EPMC12714908 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature