Exploring <i>Chlamydia trachomatis</i> screening in Shenzhen: a cost-effective sample pooling strategy for pre-marital and pre-pregnancy populations.

ABSTRACT:

Objectives

This study evaluates the feasibility of a sample pooling strategy for Chlamydia trachomatis detection in pre-marital and pre-pregnancy screening in China, where pooled testing for CT remains limited despite its use in COVID-19 and HIV screening.

Methods

From January to May 2024, 8,142 urine samples were collected from participants in pre-marital and pre-pregnancy health checks in Nanshan District. Positive urine samples with different Ct value ranges were selected, and negative urine samples were used as diluents to simulate mixed samples. We compared the sensitivity, specificity, and other metrics between the pooling tests and individual tests. A paired t-test was used to assess the statistical significance of the mean ΔCt values.

Results

The 6-samp

SUBMITTER: Zhang L 

PROVIDER: S-EPMC12714962 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature