Long-term low-dose aspirin promotes laser-induced choroidal neovascularization through suppressing TSP-1 expression.

ABSTRACT:

Purpose

To investigate the impact of low-dose, long-term aspirin use on neovascular age-related macular degeneration (nAMD).

Methods

Adult C57BL/6J or Thbs-1^-/- mice were treated with daily aspirin (1.25 mg/kg) for 8 weeks before being subjected to laser-induced choroidal neovascularization (CNV). The animals were left for 7-10 days with continued aspirin use before the eyes were collected for further investigations. Bone marrow-derived macrophages (BMDMs) and primary retinal pigment epithelial (RPE) cells were treated with different concentrations of aspirin (1, 10, 100 μM) for two days before being subjected to LPS+IFNγ for 16 h. The expression of cytokine genes was evaluated by qRT-PCR. The concentrations of thrombospondin-1 (TSP-1) were measured by ELISA.

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SUBMITTER: Yi C 

PROVIDER: S-EPMC12714992 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature