Bioinformatic analysis of glycosylation-related genes in intervertebral disc degeneration and their roles in immune infiltration and diagnostic models.

ABSTRACT: Intervertebral disc degeneration (IDD) severely impacts patients' quality of life. Elucidating IDD's molecular mechanisms is crucial for developing effective diagnostics and therapeutics. Integrating multiple IDD gene expression datasets using bioinformatics identified 25 glycosylation-related differentially expressed genes (GRDEGs). We analyzed their biological functions and regulatory networks in IDD using GO, KEGG, GSEA, and WGCNA. An IDD diagnostic LASSO regression model was constructed and validated. Immune cell infiltration analysis using CIBERSORT/ssGSEA divided IDD samples into subtypes based on glycosylation scores, exploring the immune microenvironment's influence on heterogeneity. We explored regulatory networks involving transcription factors, miRNAs, RNA-binding proteins, and

SUBMITTER: Liu G 

PROVIDER: S-EPMC12715231 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature