Structural Dynamics and Topology of Human KCNE3 in Lipid Bilayers Studied by Site-Directed Spin Labeling and Electron Paramagnetic Resonance Spectroscopy.

ABSTRACT: KCNE3 is a member of the KCNE family and consists of a single-pass transmembrane segment that is vital for cellular function. It plays a role in modulating both activities and the function of voltage-gated potassium ion channels, including KCNQ1. KCNE3 interacts with voltage-gated potassium channels to form a complex that regulates the channel's biophysical and physiological properties. KCNE3 is found in the colon, small intestine, and certain stomach cell types. Dysfunction and hereditary mutations in KCNE3 have been linked to a number of human disorders. The structural dynamics of KCNE3 in a native membrane environment are not yet fully understood. Here, we employed electron paramagnetic resonance (EPR) spectroscopy in connection with site-directed spin labeling to study the structural d

SUBMITTER: Reynolds DB 

PROVIDER: S-EPMC12715480 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature