Functional <i>D</i>- and <i>L</i>‑Naphthalenediimide-Peptides: Microwave-Driven Synthesis, Supramolecular Aggregation, and Multiphoton Fluorescence Lifetime Imaging Microscopy in Living Cells.

ABSTRACT: We report the microwave-assisted synthesis of a novel family of peptide-linked optical imaging probes incorporating the L-[7,13] bombesin fragment (denoted L-[7,13]-BBN) as a functional building block currently used for targeting the gastrin-releasing peptide receptor (GRPR) in cancer cells. Given the importance of chirality in probe design, we synthesized and evaluated both L- and D-amino acid-substituted naphthalenediimide (NDI), namely, the monopeptide (L-3) and corresponding bis-peptide (L-4) conjugates. These bioconjugates were characterized using NMR, fluorescence spectroscopy, including excitation-emission mapping, and mass spectrometry, confirming their spectroscopic tunability, water solubility, and ability to form supramolec

SUBMITTER: Giuffrida SG 

PROVIDER: S-EPMC12715529 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature