Selective Detection of Acetylcholine against Choline and In Vivo Measurement in the Mouse Brain Using the Micropipet-Supported Liquid/Liquid Interface Electrode.

ABSTRACT: Acetylcholine (ACh) is a neurotransmitter that plays critical roles in human health and diseases. To better understand ACh signaling in the brain, developing analytical capabilities for its selective and quantitative measurement in real time is essential. While electrochemical amperometry offers exceptional temporal resolution, most in vivo electrochemical ACh sensors have limited selectivity, such as against choline (the product of ACh hydrolysis) and ascorbic acid. Here, we report a micropipet-supported ITIES (interface between two immiscible electrolyte solutions), which demonstrated the selective detection of ACh against choline, ascorbic acid, and other neurotransmitters using chronoamperometry. The detection of ACh is based on its ion transfer across a water/oil interface, which was

SUBMITTER: Xu P 

PROVIDER: S-EPMC12715621 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature