Enhancing Gene Delivery and Immune Modulation in Primary Dendritic Cells by Utilizing Composites of Iron Oxide Nanocubes and Biocompatible PLGA-PDMAEMA Polymers.

ABSTRACT: Dendritic cells (DCs) represent pivotal targets in immunotherapy, gene therapy, and vaccine delivery for major diseases including cancer, autoimmune diseases, and transplant rejection. Overcoming the challenge of efficient gene delivery via conventional nonviral molecules into primary DCs has been a persistent obstacle. Within this research, we introduce an innovative gene delivery system utilizing magnetic iron oxide nanocubes (MCs) encapsulated with a biocompatible polymer, poly-(lactic-co-glycolic acid) (PL), and a cationic polymer, poly-(2-(dimethylamino)-ethyl methacrylate) (PD), facilitated by a magnetic field. Positively charged MC-PL-PD and MC-PL-PD/PD (double coating of PD) composites were synthesized, and plasmid DNA (pMAX-GFP) attachment ensued. The nanocomposite with dou

SUBMITTER: Manipuntee C 

PROVIDER: S-EPMC12715624 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature