Peptide Nucleic Acid Probes for MicroRNA Detection: Mg²⁺ Ion Effect, Surface Hybridization, and Surface Plasmon Resonance Biosensing.

ABSTRACT: This study examines how Mg²⁺ ions affect the hybridization between surface-immobilized peptide nucleic acid (PNA) probes and microRNA targets (miR125 and miR141), which is important for the development of nucleic acid-based biosensors utilizing surface plasmon resonance (SPR). The results show that appropriate concentrations of Mg²⁺ significantly enhance microRNA hybridization with PNA probes, whereas Na⁺ does not yield similar results. Kinetic analysis demonstrated that 30 and 100 mM concentrations of Mg²⁺ facilitate the interaction between the PNA probe and its microRNA target by effectively screening the negative charges of the microRNA molecules as they approach the surface. These Mg²⁺ levels also stabilize the heteroduplexes forme

SUBMITTER: Jungbluth V 

PROVIDER: S-EPMC12715733 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature