GSK3β-mediated NRF2 degradation drives Escherichia coli-induced ferroptosis in the bovine endometrium.

ABSTRACT: The infection of the bovine endometrium by Escherichia coli often results in endometrial injury with lower fertility in the bovine industry. However, what functions does ferroptosis play during endometritis? This is not completely explained. Therefore, this study aimed to elucidate the potential mechanism of ferroptosis during endometrial injury induced by E. coli. The endometrial tissues from sixteen Holstein cows with E. coli infection were harvested for pathological examination and RNA-seq. Primary bovine endometrial epithelial cells (BEECs) were used to establish an E. coli infection model in vitro. The mechanism by which ferroptosis is involved in endometritis was explored by western blot, flow cytometry, and immunofluorescence methods. The results revealed that the injury of endometr

SUBMITTER: Yuan C 

PROVIDER: S-EPMC12715911 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature