Toll like receptor agonist effects on human CD8+ T cell activation and expression of T cell checkpoint receptors.

ABSTRACT: We have previously found that murine CD8+ T cells activated with cognate antigen increase the expression of PD-1 and other checkpoint receptors, and this can be attenuated in the presence of specific Toll-like receptor (TLR) agonists. In the current report, we sought to investigate whether TLR agonists similarly affect the expression of T cell checkpoint receptors on human CD8+ T cells and their function. Cryopreserved human peripheral blood mononuclear cells (PBMCs) containing CD8+ T cells reactive to human cytomegalovirus (CMV) or Epstein-Barr virus (EBV) were stimulated with virus-specific peptide epitopes in the presence of TLR agonists. Activation of human CD8+ T cells in the presence of TLR agonists did not further expand tetramer-positive CD8+ T cells or significantly alter the expr

SUBMITTER: Jeon D 

PROVIDER: S-EPMC12716034 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature