Analysis of the interaction of TATA-box binding protein 1 and 2 from <i>Taenia solium</i> to TATA-box: structural factors related to selectivity and affinity.

ABSTRACT: TATA-box binding protein (TBP) is a core subunit of the transcription factor TFIID and plays a pivotal role in recognizing the TATA-box in protein-coding genes, facilitating the assembly of the transcription preinitiation complex. In Taenia solium, only one TBP isoform (TsTBP1) has been previously reported. Here, we identify and characterize a second isoform, TsTBP2, using a combination of molecular biology and bioinformatics approaches. TsTBP2 shares 42% primary sequence identity with TsTBP1 and exhibits distinct expression patterns between cysticerci and adult stages. To investigate the molecular determinants of DNA recognition, selectivity, and binding affinity, we performed molecular docking and molecular dynamics simulations for both TsTBPs with various TATA-box sequences. Our

SUBMITTER: Rodriguez-Lima O 

PROVIDER: S-EPMC12716038 | biostudies-literature | 2025 Aug-Oct

REPOSITORIES: biostudies-literature