Bacteria enable tolerance to bile salt exposure in an immune-competent human intestinal model.

ABSTRACT: Bile salts (BS) are known to be highly important in modulating bacteria-host crosstalk in the gut, serving as essential signaling molecules governing intestinal homeostasis. However, understanding of this crosstalk is limited by challenges of analyzing the mucosal interface in vivo and a lack of in vitro intestinal models incorporating bile, bacteria, and host cells. In this study, the impact of bile micelles on an in vitro intestinal model integrating commensal microbes with immune-competent human duodenal epithelium was studied. Physiological concentrations of BS/phosphatidylcholine (PC) micelles comprising a model bile were damaging to epithelial cells under hypoxic but not normoxic conditions. However, incorporation of a commensal bacterial consortium protected the

SUBMITTER: Li Y 

PROVIDER: S-EPMC12716047 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature