Impact of bacterial culture medium on composition and characteristics of <i>Burkholderia pseudomallei</i> extracellular polymeric substances.

ABSTRACT: Extracellular polymeric substances (EPS) are essential for maintaining the structural integrity and function of biofilms. In this study, the influence of nutrient composition on biofilm formation and EPS production by Burkholderia pseudomallei was assessed using a quantitative assay after cultivation in brain heart infusion (BHI), Luria-Bertani (LB), and modified Vogel and Bonner medium (MVBM) for 2, 4 and 6 days. Biofilm biomass, the percentage of EPS in the biofilm matrix, and the biochemical composition of EPS were analyzed. The functional groups of EPS were characterized using Fourier transform infrared (FTIR) spectroscopy, while the microstructural features of the EPS were examined using scanning electron microscopy. We found that B. pseudomallei cultured in MVBM exhibit

SUBMITTER: Srithabut S 

PROVIDER: S-EPMC12716132 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature