Bioassembly of Region-Specific Fibrocartilage Microtissues to Engineer Zonally Defined Meniscal Grafts.

ABSTRACT: Meniscal injuries are common orthopaedic problems which can impair knee function and lead to the development of osteoarthritis. While recent advances in tissue engineering have enabled the fabrication of meniscus-like grafts, these do not fully replicate the zonal structure and composition of the native meniscus. Here, fibrocartilage microtissues are used as building blocks to biofabricate zonally defined meniscal grafts. Meniscus progenitor cells (MPCs) isolated from the inner (iMPC) and outer (oMPC) regions of caprine menisci are used to engineer region-specific meniscal microtissues. Both iMPC and oMPC-derived microtissues are rich in glycosaminoglycans (GAGs) and collagen, with iMPC-derived microtissues staining more intensely for type II collagen. These microtissues are assembled into

SUBMITTER: Kronemberger GS 

PROVIDER: S-EPMC12716190 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature