Combining Nanoscale Curvature and Polymer Osmotic Pressure for Efficient Giant Vesicle Assembly Under Physiological Conditions.

ABSTRACT: Thin film hydration methods are widely used to assemble giant unilamellar vesicles (GUVs), but their efficiency drops sharply in solutions of physiological ionic strength due to enhanced adhesion between stacked lipid bilayers, which suppresses bud and foam-like mesophase formation. Here, polymer-coated nanocellulose paper (PCP) is introduced as a nanostructured substrate that overcomes this limitation. Hydration of lipid films on PCP, termed PCP-assisted hydration, achieves high GUV yields across a broad temperature range (4 °C-45 °C) using diverse soluble polymers, including ultralow-gelling agarose, hyaluronic acid, dextran, carrageenan, and polylysine. The nanoscale curvature of the cellulose fibers and the osmotic pressure generated by polymer dissolution act synergistically to promot

SUBMITTER: Cooper A 

PROVIDER: S-EPMC12716198 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature