A Modular Perfusion Bioreactor Platform for Simulating Bone Regeneration and Fracture Healing: Integrating Mechanical Loading and Dual Perfusion for Advanced In Vitro Models.

ABSTRACT: Recent advancements in tissue engineering have led to sophisticated in vitro models that better replicate physiological conditions. Bone regeneration remains a key research area due to its complex remodeling and biomechanical properties. Traditional models often fail to capture these dynamics, limiting their translational potential. Here, a modular bioreactor platform designed to simulate bone homeostasis and disease states with integrated mechanical load simulation is presented, featuring a 3D-printed microfluidic chamber, dynamic dual perfusion, and a mechanical compression device, enabling precise control of environmental parameters via a web interface. Applied to an in vitro fracture healing model, the setup prolonged viability by facilitating the inflammatory-to-anti-inflammatory tran

SUBMITTER: Pfeiffenberger M 

PROVIDER: S-EPMC12716203 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature