Serial Chemical Crystallography for Autonomous Quantitative Phase Analysis in an Electron Microscope.

ABSTRACT: We present serial electron diffraction with tilt (t-SerialED), a method for fast autonomous phase and structural analysis of beam-sensitive, nano-sized polycrystalline materials. Unlike traditional workflows collecting datasets crystal by crystal, t-SerialED acquires datasets using a batch-by-batch approach, which speeds up the data acquisition. t-SerialED combines robust indexing from 3D reciprocal space with still-shot integration and merging methods from serial crystallography. t-SerialED enables high-throughput analysis of beam-sensitive, multi-phase mixtures across a wide range of materials, from nanoporous frameworks to pharmaceutical compounds. By resolving key challenges in serial crystallography such as indexing and preferred orientation, this method enables precise structure dete

SUBMITTER: Yang T 

PROVIDER: S-EPMC12716211 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature