Glutaredoxin 2 Protects Lens Epithelial Cells From Ferroptosis by Preventing HNRNPA2B1 S-Glutathionylation in Diabetes-Mediated Cataractogenesis.

ABSTRACT:

Purpose

The purpose of this study was to investigate the role of glutaredoxin 2 (Grx2) in regulating ferroptosis under oxidative stress conditions induced by high glucose in lens epithelial cells (LECs) and its potential contribution to diabetes-mediated cataractogenesis.

Methods

Liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used to investigate proteins S-glutathionylation under high glucose condition, with validation by co-immunoprecipitation (Co-IP). Lentiviral transduction, Western blotting, FerroOrange, reactive oxygen species (ROS), and glutathione (GSH) measurements were applied to elucidate the downstream mechanisms.

Results

High glucose-induced oxidative stress triggered ferroptosis in the LECs, thereby accelerating diabetes-mediated cataractog

SUBMITTER: Li X 

PROVIDER: S-EPMC12716445 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature