Selective expansion of T-cell receptor engineered T cells with increased stem-like phenotypes using neoantigen stimulation.

ABSTRACT:

Background

Adoptive transfer of T-cell receptor-engineered T cells (TCR-T cells) has shown promising efficacy in solid tumor treatment, but achieving clinical benefit typically requires infusion of tens of billions of cells. The commonly used rapid expansion protocol (REP), based on the CD3-agonistic OKT3 antibody and irradiated allogeneic feeder cells, exponentially expands tumor-infiltrating lymphocytes (TILs). However, the effect of REP on TCR-T cell frequency and phenotype remains unclear. This study aimed to evaluate the impact of REP on TCR-T cells and to assess the potential of a neoantigen-specific stimulation platform, NeoExpand, as a strategy to selectively expand TCR-T cells with favorable phenotypes.

Methods

We compared the effects of REP and NeoExpand on the fre

SUBMITTER: Kim SP 

PROVIDER: S-EPMC12716566 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature