MigS, a cis-acting site that affects bipolar positioning of oriC on the Escherichia coli chromosome.

ABSTRACT: During replication of the Escherichia coli chromosome, the replicated Ori domains migrate towards opposite cell poles, suggesting that a cis-acting site for bipolar migration is located in this region. To identify this cis-acting site, a series of mutants was constructed by splitting subchromosomes from the original chromosome. One mutant, containing a 720 kb subchromosome, was found to be defective in the bipolar positioning of oriC. The creation of deletion mutants allowed the identification of migS, a 25 bp sequence, as the cis-acting site for the bipolar positioning of oriC. When migS was located at the replication terminus, the chromosomal segment showed bipolar positioning. migS was able to rescue bipolar migration of plasmid DNA containing a mutation in the SopABC partitioning syste

SUBMITTER: Yamaichi Y 

PROVIDER: S-EPMC1271666 | biostudies-literature | 2004 Jan

REPOSITORIES: biostudies-literature