Evidence for loss of contractile phenotype of the mouse aortic vascular smooth muscle (MOVAS) cell line with increasing number of passages in vitro.

ABSTRACT:

Aim

Vascular smooth muscle cells (VSMCs) are characterized by a considerable plasticity. Their phenotypic switch (from contractile to synthetic) plays a crucial role in the atherosclerotic process, explaining that numerous studies focus on this phenotypic transition. Thus, it is essential to use VSMCs that have been finely phenotyped for experimental purposes. The use of MOVAS cell line is suitable because, unlike primary cells, it is believed that these cells retain their phenotype and avoid cell senescence in culture. This study aimed to assess the phenotype of MOVAS cells over culture passages to ensure that they retained a contractile phenotype, before using them for further investigations.

Methods

The phenotype of MOVAS cells at different culture passages (P3, P5 and P8

SUBMITTER: Cadoret L 

PROVIDER: S-EPMC12716690 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature