Engineering N-acyl-homoserine lactone-based quorum-sensing circuit for dynamic regulatory control in Saccharomyces cerevisiae.

ABSTRACT: The yeast Saccharomyces cerevisiae is widely employed in industrial biotechnology for chemical and pharmaceutical production. However, engineering yeast for high product titre remains challenging due to metabolic imbalances and competition for cellular resources. To address this, we developed an orthogonal quorum-sensing (QS) system based on N-acyl-homoserine lactones (AHLs) for cell density-dependent regulation in yeast. Using metabolic engineering, we established AHL production in yeast. Next, we improved AHL-biosensors via directed evolution and a novel growth-based screening strategy with amdS as a counter-selectable marker. We identified three LuxR variants with enhanced sensitivity and confirmed N86 to play an important role in their sensitivity to ligands, corroborating literature o

SUBMITTER: van Aalst ACA 

PROVIDER: S-EPMC12717128 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature