Integrative analysis of pancreatic microbiota and metabolome in patients with pancreatic ductal adenocarcinoma.

ABSTRACT: The importance of microbiota-associated metabolites in pancreatic ductal adenocarcinoma (PDAC) tumorigenesis highlights the need to investigate the microbiota and metabolome along the normal-adjacent-carcinoma sequence to explore their mechanistic implications in PDAC tumorigenesis. We characterized the pancreatic microbiota and metabolome in four distinct tissue groups using 16S rRNA amplicon sequencing and untargeted metabolomics: tumor tissues from 23 PDAC patients (PDAC T), their matched para-carcinoma tissues (PCT), tumor tissues from 13 pancreatic benign tumor patients (Control T), and their matched non-tumor tissues (Control N). For the selected metabolites, we verified their functional activities using cell proliferation assay. From Control N, through PCT, to PDAC T, there were 90

SUBMITTER: Zhu Y 

PROVIDER: S-EPMC12717426 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature