Development and Application of an Antigen Capture ELISA for the Detection of Enzootic Nasal Tumor Virus-2.

ABSTRACT: Enzootic nasal tumor virus (ENTV) is the etiological agent responsible for enzootic nasal adenocarcinoma (ENA), a chronic and contagious disease predominantly affecting sheep and goats. ENTV is classified into two distinct types: ENTV-1, which infects sheep, and ENTV-2, which infects goats. ENA has been globally reported in small ruminant-rearing regions, causing significant mortality and substantial economic impacts in affected flocks. There is currently no standardized detection method for ENA. In this study, we successfully generated a monoclonal antibody (mAb) and a polyclonal antibody (pAb) against the ENTV-2 capsid protein (p27), and identified the epitope of the mAb, which was found to be highly conserved among different ENTV-2 isolates. An antigen capture ELISA (acELISA) was then s

SUBMITTER: Zhao Y 

PROVIDER: S-EPMC12717442 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature