Enhancing whole genome DNA amplification of Plasmodium falciparum for advanced molecular surveillance in malaria control.

ABSTRACT:

Background

Genomics is critical for malaria control and elimination by providing population-level monitoring of malarial gene flow. Whole genome sequencing is valuable for identifying such genomic changes and antimalarial drug resistance. However, sequencing submicroscopic and asymptomatic cases presents challenges due to their low parasite densities and relative abundance of human DNA. Selective whole genome amplification (SWGA) is a method that employs oligonucleotide primers and phi29 DNA polymerase to specifically amplify Plasmodium DNA. SWGA has proven effective in generating increased genome coverage for various Plasmodium species. Despite its promise, current SWGA protocols have proven unsuitable in routine clinical settings. This study optimizes the SWGA protocol to improve

SUBMITTER: Tan MKI 

PROVIDER: S-EPMC12717693 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature