Entropy-driven signal amplification integrated with machine learning in multiplex lateral flow immunoassay for sensitive Point-of-Care colon cancer diagnosis.

ABSTRACT: Investigations on epithelial-mesenchymal transition (EMT) events occurring on circulating tumor cells (CTCs) are poised to significantly advance nanoliquid biopsy methodologies. This study presented a colorimetric multiplex lateral flow immunoassay strip (mLFIA) for the simultaneous detection of EpCAM and Vimentin, two representative EMT biomarkers, on heterogeneous CTCs. The assay utilized an engineered magnetic probe (pCF-Apt-H1/H2), characterized by a zwitterionic polymer coating on Fe₃O₄ nanoparticles, which conferred anti-fouling properties. This probe also incorporated dual aptamers specific for EpCAM and Vimentin for recognition, along with two complementary strands (H1/H2) that enabled fluorescent detection. Upon recognition, a cascade of strand displacement a

SUBMITTER: He S 

PROVIDER: S-EPMC12717704 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature