MiR-328-5p/BCL7A axis is involved in fracture healing by modulating osteoblast function.

ABSTRACT:

Background

Delayed fracture healing severely impacts patients' quality of life and imposes economic burdens.

Objective

This study aimed to investigate the role of miR-328-5p in fracture healing and its underlying molecular mechanisms, providing novel insights for the management of delayed fracture healing.

Methods

Serum miR-328-5p levels were measured by RT-qPCR in 125 tibial fracture patients. The diagnostic potential of miR-328-5p for delayed healing was assessed through ROC curve analysis. Osteogenic differentiation of hFOB 1.19 cells was induced using osteogenic medium followed by evaluation of osteogenic markers and alkaline phosphatase (ALP) activity. Downstream targets of miR-328-5p were predicted via bioinformatics analysis and validated by dual-luciferase rep

SUBMITTER: Han Z 

PROVIDER: S-EPMC12717756 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature