Functional analysis of human induced pluripotent stem cell-derived cardiac tissue during endothelial cell network changes.

ABSTRACT:

Introduction

Tissue structure stability is essential for effective contractile function in cardiac tissue. Although endothelial cells (ECs) serve as critical sources for functional bioengineered cardiac tissue, the process of network changes in ECs affects the cardiac tissue structure. However, how the process precisely influences the synchrony of cardiomyocytes (CMs) contraction and the contraction regularity in the cardiac tissue remains unclear.

Methods

Human induced pluripotent stem cell-derived CMs (hiPSC-CMs) were cultured in well-plates for 9-10 days until they displayed stable spontaneous beating. Human umbilical vein endothelial cells (HUVECs) and normal human dermal fibroblasts (NHDFs), or NHDFs alone, were then seeded and cultured with hiPSC-CMs for 3 days (NHDF +

SUBMITTER: Koike T 

PROVIDER: S-EPMC12717779 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature