Differential functional interplay of TOGp/XMAP215 and the KinI kinesin MCAK during interphase and mitosis.

ABSTRACT: XMAP215/TOGp family members and KinI kinesins are conserved microtubule (MT)-regulatory proteins, and have been viewed as possessing prominent antagonistic stabilizing/destabilizing activities that must be balanced. Here, interdependencies between TOGp and the KinI kinesin MCAK were analyzed in human leukemia cells. A system was established that permits inducible overexpression in homogeneous cell populations that simultaneously synthesize interfering short hairpin RNAs. We present evidence that the functional interplay of TOGp and MCAK proteins is manifested as three distinct phenotypes during the cell cycle. The first involves a role for TOGp in protecting spindle MTs from MCAK activity at the centrosome, which appears essential to prevent the formation of disorganized multipolar spindle

SUBMITTER: Holmfeldt P 

PROVIDER: S-EPMC1271808 | biostudies-literature | 2004 Feb

REPOSITORIES: biostudies-literature